Acupuncture Research. 2018(08) Page:495-500Core

Electroacupuncture Relieves Obesity by Up-regulating PGC-1 α/UCP-1 Signaling in White Adipose Tissue in Diet-induced Obesity RatsChinese Full Text

WANG Li-hua;LI Jia;HUANG Wei;WANG Li;RAN Guo-ping;CHENG De-jun;ZHOU Zhong-yu;Institute of Acupuncture and Moxibustion,Hubei University of Traditional Chinese Medicine;Department of Acu-moxibustion,Hubei Provincial Hospital of Traditional Chinese Medicine;

Abstract：Objective To observe the effect of electroacupuncture（EA）on the activities of peroxisome proliferatoractivated receptor gamma coactivator-1 alpha/uncoupling protein-1（PGC-1α/UCP-1）signaling pathway in white adipose tissue（WAT）of diet-induced obesity（DIO）rats,so as to reveal its underlying mechanism in body weight loss.Methods Thirty-four male Wistar rats were randomly divided into normal diet（control,n=10）,high fat diet（model）,sham EA-acupoint and EA groups（n=8 in each of the latter 3 groups）.The obesity model was established by feeding the rats with high fat diet containing lard oil,sugar,albumen powder,cholesterol,salt and sodium cholate for 12 weeks.EA（2 Hz/15 Hz,1 mA）was applied to bilateral"Zusanli"（ST 36）and "Tianshu"（ST 25）or sham acupoints（about 5 mm beside ST 36 and ST 25）for 30 min,once daily,5 times per week for a total of 8 weeks.During the treatment,all rats were fed with normal diet,and their body weight and length were measured once a week for calculating the Lee’s index.The contents of serum total cholesterol（TC）and triglyceride（TG）were measured by using biochemical methods.The immunoactivity of PGC-1αand UCP-1 in the abdominal WAT was detected by immunohistochemistry.Results After modeling,the Lee’s index,serum TC and TG contents were significantly increased,and the levels of serum HDL-C,and PGC-1αand UCP-1 immunoactivity in WAT considerably decreased in the model group relevant to the control group（P＜0.05）.Following the treatment,the Lee’s index,TC and TG contents were significantly down-regulated while HDL-C and PGC-1αand UCP-1 immunoactivity were obviously up-regulated in the EA-acupoint group relevant to the model group（P＜0.05）.Conclusion EA can effectively reduce the body weight and adipose content in obesity rats,which may be closely related to its effect in up-regulating PGC-1α/UCP-1 signaling in WAT,suggesting an efficacy of EA in promoting the browning of WAT.

Keywords：

Electroacupuncture; Obesity; White adipose tissue; PGC-1α; UCP-1;

DOI：
10.13702/j.1000-0607.170744
Series：
Subject：
Classification Code：
R245

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Electroacupuncture Relieves Obesity by Up-regulating PGC-1 α/UCP-1 Signaling in White Adipose Tissue in Diet-induced Obesity RatsChinese Full Text

WANG Li-hua;LI Jia;HUANG Wei;WANG Li;RAN Guo-ping;CHENG De-jun;ZHOU Zhong-yu;Institute of Acupuncture and Moxibustion,Hubei University of Traditional Chinese Medicine;Department of Acu-moxibustion,Hubei Provincial Hospital of Traditional Chinese Medicine;

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