Acta Phytopathologica Sinica. 2019, (01) Core

Detection and analysis of the double-stranded RNAs diversity from Botryosphaeria dothidea strains in Hubei provinceChinese Full Text

LUO Hui;HU Wang-cheng;WANG Guo-ping;WANG Li-ping;The Key Laboratory of Plant Pathology of Hubei Province,Huazhong Agricultural University;College of Plant Science and Technology,Huazhong Agricultural University;

Abstract: Some mycoviruses have effect on growth rate,sporulation ability,pathogenicity biological characteristics of fungi host. Aims at detection of dsRNA mycoviruses infecting Botryosphaeria dothidea strains collected from Hubei province,which are to be explored as bio-control resources. The obtained results are the followings.13 strains were isolated and cultured from pear branches with symptoms of ring spot disease to some extent in Wuhan city of Hubei province. The growth rate showed differences among 13 B. dothidiea strains,which exhibit different virulence. The patterns of dsRNAs were detected. The results show that 12 B. dothidiea strains have different sizes of molecular weight. The dsRNA profiles analysis indicate that the dsRNAs from HBWH-14 strain contain 9 bands with a molecular weight range of 1-7 kb,meanwhile the other strains exhibit one or more than dsRNA bands in accordance with that of HBWH-14 strain. In total,single or mixed infection with BdCV1,BdPV1,BdRV1 and a new member of Totiviridae（ designated as BdTV1） are positive for the above one or more strains by RT-PCR detection; There was no correlation between mycoviruses and pathogenicity for 13 strains.dsRNAs from HBWH-14 regenerated strains are 100% positive by protoplast isolation.

Keywords：

Pear ring spot disease; Botryosphaeria dothidea; mycovirus; dsRNA; Botryosphaeria dothidea Chrysovirus 1（BdCV1）; Botryosphaeria dothidea Partitivirus 1（BdPV1）;

DOI：
10.13926/j.cnki.apps.000366
Series：
Subject：
Classification Code：
S436.612

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Detection and analysis of the double-stranded RNAs diversity from Botryosphaeria dothidea strains in Hubei provinceChinese Full Text

LUO Hui;HU Wang-cheng;WANG Guo-ping;WANG Li-ping;The Key Laboratory of Plant Pathology of Hubei Province,Huazhong Agricultural University;College of Plant Science and Technology,Huazhong Agricultural University;

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