Molecular Identification Technology of Parents of Hybrid Rice Based on Its Hybrid SeedChinese Full Text

YANG Zhi-gang1,2,XING Jun-jie1,2,ZHAN Qing-cai3,ZHU Ke-yong3,WANG Wei-ping1,LIU Fang-fang1,HE Zhao-yun1,YUAN Long-ping1,CAO Meng-liang1,2,*(1.China National Hybrid Rice R＆D Center,Changsha,Hunan 410125,China; 2.Changsha Hybrid Rice Genetic Engineering R＆D Center,Changsha,Hunan 410205,China; 3.Hunan Rice Research Institute,Changsha,Hunan 410125,China)

Abstract: According to the distinguishing features that seed husk has the same genotype as the female parent and SSR markers are co-dominant,a molecular technology was proposed to identify the parents of hybrid rice based on its hybrid seed.Firstly,the fingerprinting of the female parent was obtained from the husk of hybrid seed.The fingerprinting of the male parent could be deduced by comparison of the band patterns between the female and its F1 hybrid.On the basis of the existing fingerprinting bank of hybrid rice parents.It could be easy to identify whether a parent is a new one or not.We tested successfully the identification technology by using Jinyou 207 and its parents as the materials and 5 sets of primers.

Keywords：

hybrid rice seed; husk; SSR marker; molecular identification of parents;

DOI：
10.16267/j.cnki.1005-3956.2009.02.032
Series：
Subject：
Classification Code：
S511

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