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Pathological Changes in Andrias davidianus Infected with Chinese Giant Salamander Ranavirus

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【作者】 Yi GENGMatthew James GRAYKaiyu WANGDefang CHENPing OUYANGXiaoli HUANGChangliang HEZhijun ZHONGZexiao YANG

【Author】 Yi GENG;Matthew James GRAY;Kaiyu WANG;Defang CHEN;Ping OUYANG;Xiaoli HUANG;Changliang HE;Zhijun ZHONG;Zexiao YANG;College of Veterinary Medicine,Sichuan Agricultural University;Center for Wildlife Health,University of Tennessee;Department of Aquaculture,Sichuan Agricultural University;

【机构】 College of Veterinary Medicine,Sichuan Agricultural UniversityCenter for Wildlife Health,University of TennesseeDepartment of Aquaculture,Sichuan Agricultural University

【摘要】 Chinese giant salamander ranavirus(CGSRV) is an emerging pathogen in captive populations of the Chinese giant salamander(Andrias davidianus). We processed 140 morbid Chinese giant salamanders from seven captive breeding populations over five years, and describe the disease associated with CGSRV infection. The most common gross signs were significant swelling of the legs and coelomic cavity, erythema of the legs and ventrum in juveniles; cutaneous erosions and ulcerations in adults, particularly the limbs and the head; and marked petechial or ecchymotic hemorrhages of the internal organs, particularly the liver, spleen and kidney. Histological examination showed degeneration, necrosis, and inflammation in many organs, particularly in the organs where hemorrhage was observed. There was evidence of eosinophilic inclusion bodies in degenerated and necrotic cells. We identified virus particles and empty capsids without viral nucleoid in the inclusion bodies using electron microscopy. Virus particles were hexagonal or round shape, and appeared in paracrystalline arrays, aggregates, or singly. All enveloped viral particles were 140–160 nm. Polymerase chain reaction followed by sequencing verified that the virus particles were CGSRV. These results collectively support that CGSRV was the etiologic agent responsible for these mass die-offs of the Chinese giant salamander. The pathology described herein will be useful in diagnosing cases of ranaviral disease caused by CGSRV, and provide evidence that this pathogen is a significant threat to the Chinese giant salamander.

【基金】 supported by the Sichuan Technology Support Planning (No.2014NZ0027);Sichuan Academic Leader Training Fund (No.2015RST0016)
【所属期刊栏目】 ORIGINAL ARTICLE (2016年04期)
  • 【DOI】10.16373/j.cnki.ahr.160030
  • 【分类号】S947.3
  • 【被引频次】1
  • 【下载频次】14
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