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A Robust Noninvasive Approach to Study Gut Microbiota Structure of Amphibian Tadpoles by Feces

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【作者】 Xiaowei SONGJinghan SONGHonghong SONGQi ZENGKeke SHI

【Author】 Xiaowei SONG;Jinghan SONG;Honghong SONG;Qi ZENG;Keke SHI;College of Life Sciences, Xinyang Normal University;Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University;

【机构】 College of Life Sciences, Xinyang Normal UniversityInstitute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University

【摘要】 The 16S rDNA amplicon high-throughput sequencing technique provides a robust and inexpensive approach to detect the gut microbiota of amphibians. Since different experimental protocols generate technical biases in drawing the gut microbiota profiles, the integrative analysis of gut microbiota produced by different studies must be performed with circumspection. In this study, we compared the efficacy of two DNA extraction methods(i.e., a phenol-chloroform method and TIANamp Stool DNA Kit) in describing intestinal and fecal bacterial communities of transplanted Asiatic toad(Bufo gargarizans) tadpoles. In terms of the DNA extraction quality(i.e., DNA purity and yield rate) and the consistency in between fecal and intestinal microbiota structures(i.e., α and β diversity indices), the phenol-chloroform method was more robust than this commercial stool kit in profiling gut microbiota of tadpoles with feces.

【基金】 supported by the National Natural Science Foundation of China (NSFC 31600104);Key Scientific Research Project of Higher Education in Henan Province (No. 17B180004);National Undergraduate Training Program for Innovation and Entrepreneurship (No. 201610477013);Ph.D. Research Startup Foundation of Xinyang Normal University (No. 0201424);Nanhu Scholars Program for Young Scholars of Xinyang Normal University
【所属期刊栏目】 ORIGINAL ARTICLE (2018年01期)
  • 【DOI】10.16373/j.cnki.ahr.170062
  • 【分类号】Q93;Q95
  • 【下载频次】7
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