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Molecular Characterization of Thyroid Hormone Receptors (TRs) and their Responsiveness to T3 in Microhyla fissipes

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【作者】 Lusha LIUXungang WANGMengjie ZHANGJianping JIANG

【Author】 Lusha LIU;Xungang WANG;Mengjie ZHANG;Jianping JIANG;Chengdu Institute of Biology, Chinese Academy of Sciences;University of Chinese Academy of Sciences;College of Life Sciences, Nanjing Normal University;

【机构】 Chengdu Institute of Biology, Chinese Academy of SciencesUniversity of Chinese Academy of SciencesCollege of Life Sciences, Nanjing Normal University

【摘要】 To explore and enrich the molecular mechanisms of thyroid hormone receptors(TRs) in the metamorphosis of amphibians, the c DNA sequences of TRα and TRβ in Microhyla fissipes were cloned and characterized. TRα was 1 706 bp in length with an open reading frame(ORF) of 1 257 bp encoding a predicted protein of 418 amino acids and TRβ was 1 422 bp with an ORF of 1 122 bp encoding a predicted protein of 373 amino acids. Their protein sequences contained 4 conserved domains of the nuclear receptor superfamily with two highly conserved cysteine-rich zinc fingers in the DNA-binding domain, whereas TRβ was 42 amino acids shorter in its A/B domain than TRα. Highly-conserved sequences and structures indicated their conserved functions during metamorphosis. TRα expression reached peak at 12 h and then decreased from 12 h to 48 h. While dramatically up-regulated TRβ was observed after exposure of T3 within 24 h, and it was down-regulated from 24 h to 48 h. The expression pattern of TRβ is similar to that in the natural metamorphosis. Furthermore, tadpoles treated 24 h also resembled the climax of metamorphosis tadpoles and TRβexpression had higher responsiveness than TRα to T3 in M. fissipes. These results suggest M. fissipes may serve as the model to assay environmental compounds on TH signaling disruption.

【基金】 funded by the Important Research Project of Chinese Academy of Sciences (KJZG-EW-L13);2015 Western Light Talent Culture Project of the Chinese Academy of Sciences (Y6C3021);the Basic Application Project of Sichuan Province (2017JY0339)
【所属期刊栏目】 ORIGINAL ARTICLE (2018年01期)
  • 【DOI】10.16373/j.cnki.ahr.170091
  • 【分类号】Q953
  • 【下载频次】4
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