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Effect of IR Injury on Mitochondrial Proteins in Heart

Tatarkova ZuzanaLichardusova LuciaPilchova IvanaDibdiakova KatarinaCaplakova VeronikaHnilicova PetraKolisek MartinRacay PeterKaplan Peter

JFM in Martin-CU in Bratislava Slovakia

摘要:Mitochondrial reactive oxygen(ROS) and nitrogen(RNS) species have emerged as an important mechanism in cardiovascular system under basal or pathological conditions.Heart mitochondria of adult rats were divided into the control,ischemic(Isch),IR group.ROS production is primarily mediated by respiratory chain electron leakage.Activity of respiratory complexes I,II and IV significantly decreased in Isch and IR group,but complex III did not changed.Ischemia induced decrease in total antioxidant capacity,MnSOD,GSH and catalase activity with recovery in some extent during reperfusion).While MnSOD protein content was higher in IR group,activity returned to 95%of control.Nitric oxide is one of the biological molecules that can out compete MnSOD for superoxide and produce peroxynitrite.This process is faster than dismutation and led to the 10-fold higher production of nitrotyrosine after IR injury.2 D electrophoresis revealed 140 mitochondrial proteins,12 of them with significant changes after IR injury.Western blot analysis revealed 36 individual mtrotyrosine-modified proteins further identified by mass spectrometry.Linking these two groups,5 proteins were altered after IR as well as nitrated,but only one showed massive nitration per lowering content of protein after IR injury.
会议名称:

BIT’s 10th Anniversary of Protein & Peptide Conference-2017

会议时间:

2017-03-22

会议地点:

Fukuoka, Japan

  • 专辑:

    医药卫生科技

  • 专题:

    心血管系统疾病

  • 分类号:

    R54

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