PDL1 Expression and Tumor Infiltrating Lymphocyte Subset Analysis in Primary Esophageal Cancer Tissue
Tomoya SudoAkihiko KawaharaAtsushi MizoguchiHaruhiko HinoKouhei SaishoRyousuke NishidaSachiko NagasuTaizan MinamiNaoki MoriSatoru MatonoToshiaki TanakaYoshito Akagi
摘要：[Background]Tumor Infiltrating Lymphocyte（TIL） is good indicator for predicting the prognosis of esophageal cancer patients however the locoregional lymphocyte subset and its’ correlation is not well investigated.The purpose of this study is to reconfirm the clinical importance of TIL in esophageal squamous cell carcinoma（ESCC）.Secondary purpose is to investigate what type of lymphocyte subset is permeating the lesion and to assess the relationship between PDL1 expression and lymphocyte subset.[Materials and Methods]TIL was evaluated using tumor tissue and statistical analysis was performed according to the criteria of TIL working group.Immunohistochemical（IHC） analysis was performed to investigate the expression of CD3,CD8,FOXP3,CD45 RO,PDL1,HLA using degital pathology.Clustering analysis was performed according to the IHC staining pattern.[Results]There was no statistical correlation between clinicopathological factor and TIL but the overall survival of TIL positive group was significantly better than that of TIL negative group.The statistical analysis shown that TIL was an independent prognostic factor.Correlation of the subset of lymphocyte was significantly associated with each other including PDL1 and HLA.ESCC cases were divided into 3 groups by clustering analysis.Group 1 showed entirely weak expression of lymphocyte subset in and around the tumors.Group2 showed strong expression of CD8 and CD45 RO in and around the tumor.Group3 showed relatively high expression FOXP3 and low expression of CD8,CD45 RO.Group2 tended to be better prognosis and Groupl showed worse prognosis in DFS.PDL1 and HLA expression were higher in Group2.
BIT’s 2nd Annual World Congress of Digestive Disease-2017