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摘要:Introduction.One of the major mechanisms for drug resistance is associated with altered anticancer drug transport,mediated by human-adenosine triphosphate binding cassette (ABC) transporter superfamily proteins.The overexpression of MDR1 by multidrug-resistant cancer cells is a serious impediment to chemotherapy.In our study we have searched if the mechanism,with which the overexpression of MDR1 gene occurs,may be because of the structural SNPs or not.Materials and Methods.101 cases and 100 controls have genotyped with SSP-PCR (Sequence Specific Primer) then the gene expression has evaluated for 70 cases and 54 controls by Real Time PCR,for the correlation between them we secondary structures of RNA have predicted by bioinformatics tool (Vienna RNA Fold Server).Results.The frequency of ABCB1 G/G genotype in cases was 76.9%,significantly higher than 23.1% in healthy controls (p-value:0.003).While the frequency of ABCB1 A/A and A/G genotypes in healthy controls were 57.3% and 50.5% respectively,which is higher than 42.7% and 49.5% in cases (p-value:0.014).The results of Real Time PCR have shown overexpression of ABCB1 when our data has compared with each genotypes in average mode.The amount of ΔG for MDR1 mRNA is-1586.68 kcal/mol which is lower than 2482.30 kcal/mol for MDR1 mRNA in the case of rs28381943 SNP existence.Discussion.We have observed significant differences in genotypes of SNP (rs283821943) that related to overexpression of MDR1 gene.
会议名称:

2012 International Conference on System Engineering and Modeling(ICSEM 2012)

会议时间:

2012-04-07

会议地点:

Kuala Lumpur,Malaysia

  • 专辑:

    医药卫生科技

  • 专题:

    基础医学

  • 分类号:

    R346

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