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摘要:Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants.In this study,an ethyl methanesulfonate(EMS)-induced soybean population consisting of-21,600 independent M2 lines was developed.More than 1,160 M4(5)families with diverse abnormal phenotypes for seed composition,seed shape,plant morphology,and maturity that were stably expressed across different environments and generations were identified,and a mutation density of 1/12.5 kb was estimated in the mutagenized population by whole-genome resequencing.Among the mutants,a yellow pigmentation mutant displayed significantly decreased chlorophyll(Chi) content and abnormal chloroplast development.Sequence analysis showed that the leaf color mutation was allelic to that in MinnGold,in which a different SNP variation in the Mg-chelatase subunit gene(Chll1a) caused a golden yellow plant.A CAPS marker was developed and may be applied to markerassisted selection for the golden yellow phenotype in soybean breeding.The selected EMS mutants and one example of application show high potential for functional genomics research and genetic improvement in soybean.
会议名称:

第七届国际作物科学大会

会议时间:

2016-08-14

会议地点:

中国北京

  • 专辑:

    农业科技

  • 专题:

    农作物

  • 分类号:

    S565.1

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