记笔记

Development and utilization of a new ethyl methanesulfonate-treated soybean（Glycine max（L.） Merr.） population with a high mutation frequency

Zhongfeng LiLingxue JiangYansong MaZhongyan WeiHuilong HongZhangxiong LiuJinhui LeiYing LiuRongxia GuanYong GuoLongguo JinLijuan ZhangYinghui LiWei HeMing LiuNang Myint Phyu Sin HtweLin LiuBingfu GuoJian SongBing TanGuifeng LiuMaiquan LiXianli ZhangBo LiuXuehui ShiSining HanSunan HuaFulai ZhouLili YuYanfei LiLijuan Qiu

National Key Facility for Gene Resources and Genetic Improvement/ Key Laboratory of Crop Germplasm Utilization,Ministry of Agriculture,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences

摘要：Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants.In this study,an ethyl methanesulfonate（EMS）-induced soybean population consisting of-21,600 independent M₂ lines was developed.More than 1,160 M_4（5）families with diverse abnormal phenotypes for seed composition,seed shape,plant morphology,and maturity that were stably expressed across different environments and generations were identified,and a mutation density of ¹/12.5 kb was estimated in the mutagenized population by whole-genome resequencing.Among the mutants,a yellow pigmentation mutant displayed significantly decreased chlorophyll（Chi） content and abnormal chloroplast development.Sequence analysis showed that the leaf color mutation was allelic to that in MinnGold,in which a different SNP variation in the Mg-chelatase subunit gene（Chll1a） caused a golden yellow plant.A CAPS marker was developed and may be applied to markerassisted selection for the golden yellow phenotype in soybean breeding.The selected EMS mutants and one example of application show high potential for functional genomics research and genetic improvement in soybean.

关键词：

Soybean; EMS mutagenesis; Mutant population; Mutation frequency; Whole-Genome resequencing;

会议名称：

第七届国际作物科学大会

会议时间：

2016-08-14

会议地点：

中国北京