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摘要:PR60 gene,which is dominantly expressed in Endosperm Transfer Cell,encodes a lipid transfer protein(LTP).Modelling of LTP has revealed that the internal cavity encloses lipid or lipid-like molecules to or through a membrane,helping the nutrition transduction and transmission from maternal tissue,which is in accordance with the grain size in direct proportion to the PR60 expression uncovered in our research.The CDS of PR60 gene was cloned from eleven major Australian cultivars.Three gene loci have been identified by means of PCR and verified by sequencing.Deletion lines in Chinese Spring wheat were used to locate theses loci.It is revealed that three are located on chromosome 1D(L21D and L41D) and one on chromosome 1B(L51B).DNA markers have been designed and so far three alleles have been identified on locus 2(L21Da,L21 Db,and L21Dc)and locus 5(L51Ba,L51 Bb,and L51Bc),two alleles have been identified on locus 4(L41Da and L41Db).Over-expression of two loci(L21Da and L51Ba)on PR60 have been undergoing for the purpose of revealing the associated functions of agronomic important traits especially grain development.The research will focus on the allelic effect in relation to PR60 gene functions and develop potential tools for wheat breeders to improve wheat yield and quality.
会议名称:

第七届国际作物科学大会

会议时间:

2016-08-14

会议地点:

中国北京

  • 专辑:

    农业科技

  • 专题:

    农作物

  • 分类号:

    S512.1

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