Novel Applications of Microbe-Electrode Hybrid System for Bioenergy Production and Biorefinery Process
摘要：Biological production of fuels and chemicals has gained substantial importance in recent years due to fossil fuel depletion and environmental concern. However, synthesis of these bioproducts at high titer and yield are constrained by various factors, among them internal redox imbalance（generally caused by undisposed electrons） is considered as one of the major factors. The bioelectrochemical system with electrode interfaced biocatalyst provides a biotechnological platform as it could charge and discharge electrons via extracellular electron transfer. In the absence of these electron acceptors, electrons are disposed by generating large amount of undesired reduced byproducts, which seriously affects the commercial viability of these bioprocesses. Recently, Shewanella oneidensis MR-1, an electrochemically active bacterium showed promising results to regulate redox imbalance complications without either using expensive and non-sustainable electron acceptors or by producing undesirable byproducts. S. oneidensis MR-1 can intrinsically transfer electrons directly to electrodes through a process called extracellular electron transfer（EET） there by providing a promising approach to control internal redox status of the cells. In this study, we demonstrate the biological production of 3-HP by a recombinant S. oneidensis MR-1dha Bgdr AB strain grown in an Electrochemical Bioreactor Array（EBA）. The EBA equipped with DC power supply and coupled with an array circuit board can control applied potential and simultaneously investigate the effect of potential. Here, we examine recombinant S. oneidensis MR-1dha Bgdr AB as a potential electrode interfaced biocatalyst to produce 3-hydroxypropionic acid（3-HP） from glycerol, and anticipate achieving high 3-HP yield on glycerol. This study also intends to develop recombinant strains with artificial EET system and examine its potential to produce various other biofuel and biochemical at high yield.
2016 Henan-Busan International Joint Symposium on Chemistry Application
Xinyang , Henan, China