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摘要:Toxoplasma gondii is one of the most prevalent intracellular parasites and is threatening the health of both humans and animals,therefore causing incalculable economic losses worldwide.Vaccination is thought to be an efficient ways of controlling toxoplasmosis.T.gondii microneme protein 11(MIC11) is a soluble microneme protein which is presumably considered facilitating the early stage of cell invasion.To evaluate the protective efficacy of T.gondii MIC11,in the present study,a new DNA vaccine encoding the a-chain of T.gondii MIC11 was constructed using the pcDNA3.1 vector.Expression of MIC11 from this vector was confirmed by indirect immunofluorescence assay following transfection into baby hamster kidney(BHK) cells.Intramuscular immunization of BALB/c mice with pcDNA/MIC11 was carried out to evaluate the immune responses by serum antibodies titers,lymphoproliferation assay and cytokines assay.The protective efficacy was evaluated by survival rate in mice after challenging with highly virulent strain of T.gondii.The results demonstrated that this vaccination elicited significant humoral responses and T.gondii lysate antigen(TLA)-stimulated lymphoproliferation(p<0.05).Compared to controls,the pcDNA/MIC11 immunized mice had high production of IFN-γ,IL-12 and IL-2(p<0.05),but not IL-4(p>0.05),indicating that a predominant Th1 type response was developed.The vaccination also increased the survival rate of immunized mice when they were challenged with a lethal dose of tachyzoites of T.gondii RH strain.These data suggest that T.gondii MIC11 is a reasonable vaccine candidate deserving further studies and pcDNA/MIC11 is a potential strategy for the control of toxoplasmosis.
会议名称:

中国动物学会寄生虫学专业委员会第十四次全国学术会议暨第五次国际寄生虫学学术研讨会

会议时间:

2013-07-31

会议地点:

中国贵州贵阳

  • 专辑:

    医药卫生科技

  • 专题:

    基础医学

  • 分类号:

    R392

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