First genetic characterization of Toxoplasma gondii infection in poultry meat intended for human consumption in Eastern China
Lan-Bi Nie1,2Yang Zou1,2Nian-Zhang Zhang1Xiao-Jie Xu3Feng-Cai Zou4Xing-Quan Zhu1Wei Cong1,3
1. State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences2. College of Animal Science and Technology,Jilin Agricultural University3. College of Marine Science,Shandong University at Weihai4. College of Animal Science and Technology,Yunnan Agricultural University
摘要：Toxoplasma gondii can infect nearly all warm-blooded animals including birds.However,limited information on the molecular epidemiology and genotypes of T.gondii infecting poultry is available in China.Therefore,the present study characterized T.gondii genotypes in poultry meat in eastern China.During August 2015 and September 2016,muscle tissue samples collecting from 414 poultries（257 chickens,115 ducks and 42 geese） in Shandong provinces were used to detect the T.gondii B1 gene by a semi-nested PCR,and the positive samples were genotyped at 10 nuclear loci（i.e.,SAG1,alternative SAG2,5’-and 3’-SAG2,SAG3,L358,BTUB,c22-8,GRA6,c29-2,PK1） and an apicoplast locus Apico by multi-locus PCR-RFLP technology.Thirty-two（7.37%） samples from 414 poultry meat were T.gondii B1 gene positive.Chicken had the highest T.gondii prevalence（8.17%）,followed by ducks（7.83%） and geese（4.76%）.Furthermore,only one genotype（Toxo DB#9） was identified.This is the first genetic characterization of T.gondii isolates from poultry meat in Shandong province,eastern China and also the first report of genotype Toxo DB#9 was found in poultry in China,which provide basic data for the surveillance and control of T.gondii infection in poultry,other animals and humans.
基金：
Project support was provided in part by the Fundamental Research Funds of Chinese Academy of Agricultural Sciences（Grant No.Y2016JC05）； the Excellent Scientist Fund of Yunnan Agricultural University（2015JY03）； National Natural Science Foundation of China（Grant No.31230073）； the Agricultural Science and Technology Innovation Program（ASTIP）（Grant No.CAAS-ASTIP-2014-LVRI-03）；
会议名称：
中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会
会议时间：
2017-10-13
会议地点：
中国江西九江
- 专辑：
农业
- 专题：
畜牧与动物医学
- 分类号：
S855.9
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