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Detection of Mediterranean Fruit Fly Larvae Ceratitis capitata (Diptera: Tephidae) in Different Types of Fruit by HS-SPME GC-MS Method

Hasan Al-Khshemawee1,2Manjree Agarwal1Xin Du1Yonglin Ren1

1. School of Veterinary and Life Science, Murdoch University2. College of Agriculture, Wasit University

摘要:Timely detection of Mediterranean fruit fly(Medfly) is very important so that eradication action can be taken on time. The larvae stage of this insect is the most dangerous stage as it is within the pulp of the fruit, making it hard to detect by visual inspection. In most countries at ports of entry the inspector check a small sample of fruit by visual inspection or by cutting the produce and searching for fungus and pests. This paper will investigate a quick, reliable and sensitive method to determine the presence of fruit flies. Our research focuses on developing the technology for detecting hidden infestations by using the Head Space-Soild Phase Micro Extraction(HS-SPME) method coupled with Gas Chromatography-Mass Spectrocopy(GC-MS) technique. Five different types of fruit were infested with an early stage of Medfly Ceratitis capitata Wiedemann(Diptera: Tephidae). We investigated to detect the differences in volatile organic compounds(VOC’s) between infested and non-infested fruits by using HS-SPME with(GC-MS). The results indicated that for few chemicals no significant differences between infested and non-infested fruit can be seen, especially in the fruits with first instar. However, in case of third instar larvae infested fruits significant differences in the chemicals can be seen as compare to non infested fruits and other instar infestations. These chemicals include ethyl(Z)-2 butenoate, 2-heptanone, anisole, β-cis-ocimene, 1,3,7-nonatriene,4,8-dimethy-,ethyl octyate, isoamyl caproate and 1β,4βh,10βh-guaia-5,11-diene, in apple. Ethyl(Z)-2-butenoate,(+)-2-bornanone,(-)-trans-isopiperitenol, methyl caprate, caryophyllene and farnesene in orange. Butanoic acid, 3-methyl-,2-methylbutul acetate, sabinene, β-myrcene, octanoic acid, methyl ester, dihydrocarvone,(-)-trans-isopiperitenol and ethyl laurate in mandarin. Butyl 2-methylbutanoate, terpinen-4-ol, P-menth-8-en-2-one, E-,(3 E,7 E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene and dodecanoic acid, ethyl ester in lemon. Decane, 3-methyl-, p-menth-1,4(8)-diene, 1-undecene and α-cubebene in avocado. Thus, the VOC’s method couldprovide a possible tool for detecting tephritid larvae and this method could be adopted by industries importing and exporting fruit.
会议名称:

Workshop 3 2017

会议时间:

2017-03-18

会议地点:

中国陕西西安

  • 专辑:

    农业科技

  • 专题:

    植物保护

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    S433

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