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摘要:<正>Heterologous expression studies have demonstrated that the PDZ-proteins NHERF1,NHERF2 and PDZK1 (NHERF3)modulate CFTR membrane expression,conductivity and interaction with other proteins.To study their biological roles in vivo in an epithelium that highly expresses both CFTR and all three NHERF proteins,we investigated the effect of NHERF1,2 and PDZK1 ablation,or a combination of the above,on duodenal HCO3- secretion in the basal state and after agonist and inhibitor application.The proximal duodenum of anesthetized mice was perfused in situ,and HCO3- secretion was determined by back-titration.NHERF1 ablation strongly reduced basal and forskolin(FSK)-stimulated HCO3- secretory rates,and completely preventedβ2-adrenergic stimulation.NHERF2 deletion significantly augmented FSK-stimulated HCO3- secretion,prevented the inhibitory effect of LPA,and partially rescued the suppressed basal HCO3 secretion resulting from NHERF1 ablation.PDZK1 ablation reduced basal HCO3- secretion but not the response to FSK.The deletion of CFTR abolished agonist-mediated HCO3- secretion and any effect of NHERF ablation.We conclude that the three NHERF proteins differentially modulate basal and agonist -mediated duodenal HCO3 secretion in vivo in a CFTR-dependent fashion.NHERF1 is an obligatory linker forβ2-Vadrenergic stimulation of CFTR,and strongly augments cAMP-mediated stimulation.NHERF2 confers inhibitory signals,i.e.as a coupling factor between inhibitory LPA receptors and CFTR.
会议名称:

2008北京国际生理科学学术大会

会议时间:

2008-10

会议地点:

中国北京

  • 专辑:

    医药卫生科技

  • 专题:

    基础医学

  • 分类号:

    R341

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