Evaluation of phytochemicals,antimicrobial and antioxidant activities of Eclipta alba and Fagonia cretica
Fazlullah Khan1,2Mukhtiar Hassan1Shahzad Hussain3Ahmad Ali4Farnaz Malik3Kamal Niaz2
1. Department of Biochemistry, Hazara University,Garden Campus2. Department of Toxicology and Pharmacology, Faculty of Pharmacy,Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences3. National Institute of Health4. Centre for Plant Sciences and Biodiversity, University of Swat
摘要：The safety of herbal products has become famous in public health with their recognition and worldwide market growth due to the widespread hypothesis that herbal products are risk free. Therefore, phyto-therapy has been incorporated into all alternative systems of medicine, often as the main source of healthcare in developing and under developed countries. The present study was conducted to determine prominent phytochemicals, antimicrobial and antioxidant activity of Eclipta alba（E. alba） and Fagonia cretica（F. cretica） collected from different regions of Pakistan. The level of 1, 1-diphenyl-2-picryl hydrazyl, flavonoids, and phenolics were higher in both plants. The antimicrobial activity was against gram positive bacteria: Bacillus subtilis（B. subtilis）, Staphylococcus aureus（S. aureus） and gram negative bacteria: Salmonella typhi（S. typhi）, Escherichia coli（E. coli）, Vibrio cholera（V. cholera） and yeast: Sacchromyces cerevisiae（S. cerevisiae）, the ethanolic extracts of both plants showed significance result against bacteria and yeast（p<0.05） with respect to aqueous and n-hexane extracts. E. alba and F. cretica showed significantly higher antioxidant effects due to the presence of numerous phytochemicals and flavonoids（p<0.05）. For the purpose of antimicrobial activity agar well plate diffusion method was employed for aqueous, ethanolic and n-hexane extracts. The present study showed that the ethanolic extracts have antimicrobial activity against all the bacteria and yeast due to higher flavonoids and phenolics compounds while the aqueous and n-hexane have no antimicrobial activity against all the bacteria and yeast. Our study identifies plants with significant antimicrobial and antioxidant potential which can be used for the separation of useful therapeutic compounds and to formulate new infusions, nutraceuticals and pharmaceuticals.
2017 International Conference on Agricultural and Food Science （ICAFS 2017）