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摘要:N1,N12-diacetylspermine(DiAcSpm) is a diacetylated derivative of spermine.Urinary DiAcSpm levels of various cancer patients had been found to be significantly higher than those in normal people,which suggested that DiAcSpm might be a potential broad-spectrum tumor marker.In this study,anti-DiAcSpm polyclonal antibody was obtained by immunization of rabbits.8 kinds of analogous or relative molecules were employed to estimate the specificity of the antibody.The direct competitive and indirect competitive ELISAs were developed.And the sensitivity of the two assays were 7.79 ± 0.48 ng/mL(n=3) and 4.73 ± 0.67 ng/mL(n=3);the limits of detection of the two assays were 0.55 ± 0.07 ng/mL(n=3) and 0.22 ± 0.02 ng/mL(n=3),respectively.The indirect competitive ELISA was used to measure DiAcSpm in healthy people urine,and the recoveries of spiked DiAcSpm ranged from 109.96 to 117.13%.
会议名称:

2019 International Conference on Biotechnology and Bioengineering(9th ICBB)

会议时间:

2019-09-25

会议地点:

Poznan, Poland

  • 专辑:

    医药卫生科技

  • 专题:

    临床医学

  • DOI:

    10.26914/c.cnkihy.2019.067760

  • 分类号:

    R446.6

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页码:75-81 页数:7 大小:189k

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