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摘要:OBJECTIVE To evaluate the he genotoxicity and carcinogenicity potential of PM2.5 collected in a northern city of China. METHODS PM2.5 samples which were collected from a city in north of China were divided into "Heating supply" group(Nov., 2015 to Feb.,2016) and "Post-heating supply" group(Apr., 2016, to Jul., 2016) according to the heat supply period. Ames test(up to 5 mg/dish) and SOS/umu test(up to 500 μg) were used to evaluate their abilities to induce mutation or DNA damage.The oncogenic potential of the two samples was evaluated by malignant transformation assay(up to21.0 ng/cm~2) based on a human bronchial epithelial cells which is dubbed as BEAS-2 B. RESULTS Ames test and SOS/umu test showed that two groups of samples possess distinguish characteristics in genotoxicity. Samples in "heat supply" period mainly caused DNA damage, but the mutagenic property was weak; "Post-heat supply" samples showed significant mutagenicity, but were less capable of inducing DNA damage. The results of cell malignant transformation test indicates that long-term exposure(6 months) of both groups of samples could induce malignant transformation on BEAS-2 B, which is manifested in increased cell proliferation rate(population doubling time decreased by about one-third), aberrant growth pattern(anchorage-independent), resistance to apoptosis-inducing factors, cell epithelialmesenchymal transformation(including morphological change, abnormal expression of E-cadherin and other molecular markers). In addition, transcriptome studies have shown that epigenetic changes such as DNA methylation are associated with cell malignant transformation induced by PM2.5. CONCLUSION PM2.5 samples from an urban area in north of China have significant genotoxicity, and carcinogenic potential to a certain degree, but the toxicity characteristics of PM2.5 samples between "Heat supply" and "Post-heat supply" period shows great difference.
会议名称:

第九届亚洲毒理学大会暨中国毒理学会第八次中青年科技论坛

会议时间:

2021-10-20

会议地点:

中国浙江杭州

  • 专辑:

    工程科技Ⅰ辑; 医药卫生科技

  • 专题:

    环境科学与资源利用; 预防医学与卫生学

  • DOI:

    10.26914/c.cnkihy.2021.052475

  • 分类号:

    X513;R114

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