Antioxidant activity and the potential mechanism of the fruit from Ailanthus altissima Swingle
Ya-nan Mo1Feng Cheng1,,,4Zhen Yang1Xiao-fei Shang1Jian-ping Liang1Ruo-feng Shang1Bao-cheng Hao1Xue-hong Wang1Hong-juan Zhang1Ahmidin Wali4Chun-fang Lu4Yu Liu1,,,5
1. Key Laboratory of New Animal Drug Project,Gansu Province2. Key Laboratory of Veterinary Pharmaceutical Development,Ministry of Agriculture and Rural Affairs3. Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Sciences4. Key Laboratory of Plant Resources and Chemistry in Arid Regions,Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences5. College of Veterinary Medicine,Gansu Agricultural University
摘要：The fruits of Ailanthus altissima Swingle（AS） possess a variety of pharmacological activities. Its antioxidant activity and the potential mode of action have not yet been investigated. Antioxidant assay in vitro proved AS possessed fairly strong antioxidant capacity. Meanwhile, AS increased the activities of SOD, CAT, GSH-Px and decreased the MDA level compared with the H2 O2 group, suggesting it relieved oxidative stress of RAW264.7 cells significantly. Network pharmacology analysis screened the core targets of AS like threonine kinase1（AKT1）, mitogen-activated protein kinase 1（MAPK1）, sirtuin-1（SIRT1）, mechanistic target of rapamycin kinase（MTOR） and the key pathways involved PI3 K-AKT and FoxO signaling pathway. Besides, qRT-PCR revealed AS preconditioning significantly upregulated the expression level of AKT1, SIRT1, MAPK1, MTOR in model cells, and the effect was related to the regulation of Fox O and PI3 K/AKT signaling pathway. In summary, AS revealed signifi-cant antioxidant activity and its potential mechanism was regulating FoxO and PI3 K/AKT signaling pathway.