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Shotgun Sequencing And Analysis of the Macronuclear Genome of the Model Eukaryote, the Shotgun Ciliate Tetrahymena thermophila

Jonathan A. EisenMartin WuEileen HamiltonWilliam C. NiermanSusan van AkenTamara FeldblyumHean KooArthur DelcherSteven L. SalzbergBill MajorosJonathan BadgerJane CarltonJoana SilvaMaryam FarzadJennifer WortmanRoger SmithEduardo Orias

The Institute for Genomic Research 9712 Medical Center Drive,Rockville,MD 20850,U.S.A.Department of Molecular Cellular and Developmental Biology,University of California,Santa Barbara,Santa Barbara,CA 93106,U.S.A.

摘要:<正>The macronuclear (MAC) genome of Tetrahymena thermophila strain SB210, a ciliated protozoon that is a model organism for molecular and cellular studies of eukaryotes, has been sequenced to a depth of 9-fold coverage using the shotgun method. Of the two nuclear genomes in T. thermophila, we chose to sequence the MAC because it contains less repetitive DNA than the micronuclear genome and because gene expression is carried out in the MAC. The sequences were assembled into scaffolds and contigs that cover 106 megabases of DNA. We will present evidence that this likely represents most if not all of
会议名称:

12th International Congress of Protozoology

会议时间:

2005-07

会议地点:

中国广州

  • 专辑:

    理工A(数学物理力学天地生)

  • 专题:

    生物学

  • 分类号:

    Q78

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