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摘要:Background Adult cortex has long been regarded as "non-neurogenic". Recent studies have indicated the occurrence of neurogenesis in cortex after ischemic stroke. Migrating new-born neurons from the subventricular zone(SVZ) have been demonstrated to contribute to the ischemia-induced neurogenesis in cortex. However, it is still controversial whether local neurogenesis could occur. Here, we investigated this issue by genetic fate mapping, progenitor depletion, and retrovirus labeled patch clamping. Methods and Results Focal ischemia was made by photothrombosis. Co-expression of neural stem cell markers Sox2 and Pax6 with GFAP at the lesion site was found from 3 dpi. Double-staining of BrdU and DCX were observed at 7dpi. Nestin-cre ER:Rosa- DTA mice were used to explore the origin of new-born neurons. Tomoxifen(TAM) was administered either before ischemia to deplete SVZ neural stem cells or post ischemia to deplete cortical Nestin-expressing cells. No significant decrease of cortical Brd U/DCX cells were observed in mice treated with TAM before injury, while significant decrease of Brd U/DCX cells was found in mice treated with TAM after ischemia. To confirm the ischemia-induced local neurogenesis, we injected retrovirus-GFP into the injured cortex of DCX-ds Red mice, and performed whole-cell patch-clamping on dsR ed/GFP double positive cells after ischemia. Action potentials were recorded from about 90% of ds Red/GFP double-positive cells. Further, GFAP-Cre-AAV was injected into the ischemic cortex of Rosa-YFP mice to trace the contribution of astrocytes to the ischemia-induced neurogenesis. Both YFP labeled DCX-positive and Cux1-positive cells were found in the ischemic cortex. Conclusions: These results indicated that focal ischemia can induce a rapid local neurogenesis in adult mice cortex, possibly from resident nestin-positive reactive astrocytes. Our work suggests that endogenous neurogenesis may be amplified for repair after ischemia in the future.
会议名称:

第六届亚太神经科学联合会学术会议暨中国神经科学学会第十一届全国学术会议

会议时间:

2015-09-20

会议地点:

中国浙江桐乡

  • 专辑:

    医药卫生科技

  • 专题:

    神经病学

  • 分类号:

    R743

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