文献知网节

Mechanisms of calcium independent but voltage dependent secretion(CiVDS) in somata of mammalian primary sensory neurons

Yuan WangZuyin ChaiRong HuangXiaoyu ZhangRong ZhouChanghe WangZhuan Zhou

State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking University

摘要:The somata of primary sensory neurons, including dorsal root ganglion(DRG) neurons, release neurotransmitters and neuropeptides. Following physiological action potentials, in addition to Ca2+-dependent secretion, we have discovered and studied Ca2+-independent but voltagedependent secretion(Ci VDS) in somata of freshly isolated DRG neurons(Zhang et al, 2002, 2004; Zheng et al, 2009, Liu et al, 2011). Major open question of Ci VDS is the molecular mechanism, including 3 components: fusion pore machinery(FP), voltage sensor(VS) and the FP-VS linker(LK). Here we report, by using exocytosis assays of patch-clamp recording of membrane capacitance, and single vesicle imaging(EM and TIRF),(1) FP is jointly contributed by 2 components of SNARE complex, SNAP25 and syntaxin;(2) VS is contributed by voltage-gated Ca2+ channels(VGCCs), Cav2.2(N-type VGCC);(3) LK is the "synprint", Cav2.2 intracellular loop 718-963aa(Catterall, 1999);(4) following automatic knockdown of Ci VDS by 3d-culture of DRG neurons, Ci VDS is rescued by overexpressing any component of FP(SNAPE25 or syntaxin) or VS(Cav2.2);(5) Ci VDS is inhibited by blockers against FP(SNAP25 or syntaxin), VS(Cav2.2) and LK(synprint-truncated);(6) finally, Ci VDS is blocked by Cav2.2-RNAi-KD in DRG pretransfected in vivo.
会议名称:

第九届海内外华人神经科学家研讨会

会议时间:

2016-07-25

会议地点:

中国安徽合肥

  • 专辑:

    基础科学; 医药卫生科技

  • 专题:

    生物学; 基础医学

  • 分类号:

    R338

  • 手机阅读
    即刻使用手机阅读
    第一步

    扫描二维码下载

    "移动知网-全球学术快报"客户端

    第二步

    打开“全球学术快报”

    点击首页左上角的扫描图标

    第三步

    扫描二维码

    手机同步阅读本篇文献

  • CAJ下载
  • PDF下载

下载手机APP用APP扫此码同步阅读该篇文章

温馨提示:阅读CAJ格式原文,请使用CAJ浏览器

下载:1 页码:136 页数:1 大小:1820k

引文网络
  • 参考文献
  • 引证文献
  • 共引文献
  • 同被引文献
  • 二级参考文献
  • 二级引证文献
  • 批量下载
相关推荐
  • 相似文献
  • 读者推荐
  • 相关基金文献
  • 相关法规
  • 关联作者
  • 相关视频