Role of microRNAs in ginsenoside-Rg1-induced angiogenesis
Department of Biology,Hong Kong Baptist University
摘要：<正>MicroRNAs（miRNAs）are small noncoding RNAs of 19-24 nucleotides.They regulate gene expression by translational repression or mRNA degradation in a sequence-specific manner; and are thereby implicated to regulate a variety of biological processes including cell proliferation, differentiation and apoptosis.Previously,ginsenoside-Rg1,a pharmacologically active component of Panax ginseng,has been found to promote angiogenesis and wound healing in vitro and in vivo. Since angiogenesis is crucial for a variety of physiological and pathological processes such as wound healing and tumorigenesis;in this study,we used human umbilical vein endothelial cells （HUVECs）as a cellular system to investigate the role of miRNA in Rg1-induced angiogenesis.By performing miRNA microarray,the expressions of 1812 miRNA in normal and Rg1-induced HUVECs were detected.Among them,17 down-regulated miRNAs（e.g.hsa-miR-377,has-miR214 and hsa-miR-23a）and 5 up-regulated miRNAs（e.g.hsa-miR-520h and hsa-miR-197）were found to be related to the Rg1-induced angiogenesis.Subsequently,the miRNA/target gene pairs were identified by the computational analysis;and they will be further validated by the gain-of function and loss-of function experiments.We believe that this specific miRNA signature can help us to explain the pharmacogenomic actions of Rg1 by acting as an angiogenic agent.
2008 International Conference on Ginseng