The anti-aging effects of ginsenosides on endothelial cell.
Department of Biology,Hong Kong Baptist University
摘要：<正>Human aging is accompanied by degeneration of various tissues which loose part of their normal physiological functions,whereas,oxidative stress is believed to be a crucial factor in many of these degenerative processes.It is known that the increase in oxidative stress in endothelial cells during aging may lead to apoptosis or cellular senescence,and consequence in different age-related diseases such as atherosclerosis,coronary heart disease,and stroke.The present study is aimed to investigate the protective effects of ginsenosides,the major active components of ginseng, on the oxidative injured endothelial cells.Hydrogen peroxide（H2O2）,one of the major reactive oxygen species（ROS）,is known to induce cell cycle arrest,DNA damage and apoptosis in various types of cells.Our preliminary results showed that H2O2（40nM-40mM）induce cell death in human umbilical vein endothelial cells（HUVECs）in a dose dependent manner.Pre-treatment and post-treatment of the 10μM ginsenoside protopanaxatdol（PPT）for 24 hours was able to improve the cell viability after the H2O2 insult for 15%.It was also found to inhibit the percentage of apoptosis induced by HO2.Furthermore,PPT could restore 20% of the intracellular NAD+level suppressed by H2O2-induced oxidative injury.Besides,since the sirtuin genes(especially the mammalian silent information regulator 2 homolog（SIRT1）,the NAD+-dependent protein deacetylases,are function as anti-aging genes in ranging from gene silencing,regulating cell cycle and apoptosis,to energy homeostasis.Hence,SIRT1 and its decetylation target,FoxO transcriptional factor are implicated in the protective effect of PPT on the H2O2-damaged HUVECs. An understanding of how ginsenosides can protect cells against ROS-induced cell damage is important to account for the anti-aging and longevity ability of ginseng.
2008 International Conference on Ginseng