Brain opioid receptor responses to psychological stress in atopic dermatitis model mice,NC/Nga
1. Department of Dermatology,Gunma University Graduate School of Medicine2. National Institute of Health Sciences,Division of Novel Foods and Immunochemistry
摘要：＜正＞Background Atopic dermatitis is a common pruritic inflammatory skin disease.It has been well recognized that environmental factors such as mites and dusts and psychological stress exacerbate atopic dermatitis.Recently,we have demonstrated that psychological stress by itself can induce scratching behaviour and trigger atopic dermatitis-like skin changes in atopic dermatitis model mice,NC/Nga.Additionally,we have shown that the pretreatment with corticotropin-releasing factor（CRF）inhibits scratching behaviour induced by psychological stress.In this study,to explore the mechanism how psychological stress can induce itch and also how CRF inhibits scratching behaviour induced by psychological stress,we investigated the expression level of opioid receptors（u and k）mRNA in brain as well as other parameters. Methods and Results Under SPF condition,psychological stress elicited atopic dermatitis-like skin lesions along with the increased level of serum IgE in NC/Nga mice.Both u-opioid receptor antagonist and CRF suppressed scratching behaviour.Psychological stress decreased the mRNA expression of K-opioid receptors in brain,and pretreatment with CRF restored the mRNA levels of K-opioid receptors to those of control mice.On the other hand,psychological stress increased the mRNA expression of u-opioid receptors in brain,and pretreatment with CRF restored the mRNA levels of u-opioid receptors to those of control mice. Conclusion Our data indicate that CRF may suppress stress-induced scratching behaviour via through the restoration of altered expression of u and K-opioid receptors in brain.
The Second Eastern Asia Dermatology Congress（EADC2012）