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Genomewide analysis of chitinase and chitin deacetylase gene family in the oriental fruit fly, Bactrocera dorsalis(Hendel)

Shi-Huo LiuHong-Fei LiYang YangRui-Lin YangYu-Xia PeiBi-Yue DingWen-Jia YangHong-Bo JiangWei DouJin-Jun Wang

Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest UniversityAcademy of Agricultural Sciences, Southwest UniversityKey & Special Laboratory of Guizhou Education Department for Pest Control and Resource Utilization,College of Biology and Environmental Engineering, Guiyang University

摘要:Chitin is one of the most common amino polysaccharide and widely distributed in fungi, nematodes and arthropods. Chitinase(Cht) and chitin deacetylase(CDAs) are important enzymes required for chitin metabolism in insects. In this study, twelve chitinase-like genes(including seven chitinase genes and five imaginal disc growth factor genes) and six CDA genes(encoding seven proteins) have been identified in Bactrocera dorsalis through genome-wide searching and transcript profiling. Based on conserved sequences and phylogenetic relationships, twelve chitinase-like proteins were clustered into eight groups(group Ⅰ–Ⅴ and group Ⅶ–Ⅸ). And domain architecture analysis showed that all of them contained at least one glycoside hydrolase family 18(GH18) catalytic domain, however, only four(BdCht5, BdCht7, BdCht8 and Bd Cht10) contained chitin-binding domains. Phylogenetic analysis showed that these seven CDAs were clustered into five groups(group Ⅰ–Ⅴ). Domain analysis showed that all of them contained one chitin deacetylase catalytic domain, and six CDAs(except BdCDA9) contained one chitin binding domain. Developmental and tissue-specific expression pattern analysis revealed that the transcript levels of twelve chitinase-like genes and six CDA genes differed a lot from egg to adult, as well as among different tissue of larva and adult. Our investigation illustrate the structural differences and the expression patterns of chitinases and chitin deacetylases in B. dorsalis, and provided important information for further studies on the functions and developing new pest control strategies based on these vital enzymes.
会议名称:

Building Capacity to Manage Biological Invasions and Facilitate Trade--The Third International Congress on Biological Invasions(ICBI 2017)

会议时间:

2017-11-19

会议地点:

中国浙江杭州

  • 专辑:

    农业科技

  • 专题:

    植物保护

  • 分类号:

    S433

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