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Isolation and Identification of Four Strains of Salmonella and Drug Susceptibility Test

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【作者】 Guo RuiDing JialinLuo JunfengFeng LingLiu ZhiqiangZhao XiaocuiYao WeipingGao QingshanFu YanfangSu ShuoqingShi Qiumei

【Author】 Guo Rui;Ding Jialin;Luo Junfeng;Feng Ling;Liu Zhiqiang;Zhao Xiaocui;Yao Weiping;Gao Qingshan;Fu Yanfang;Su Shuoqing;Shi Qiumei;Changli Bureau of Agriculture,Forestry, Animal Husbandry and Aquatic Products;Qilu Shenghua Pharmaceutical Co., Ltd.;Animal Husbandry Bureau of Haigang District;Leting Animal Husbandry Bureau;Liushouying Animal Epidemic Surveillance Station, Agriculture and Animal Husbandry Bureau of Funing District;Animal Epidemic Prevention Station of Luanzhou Town, Luanzhou Bureau of Animal Husbandry and Aquatic Products;Hebei Animal Husbandry Station;Hebei Normal University of Science and Technology/Hebei Key Laboratory of Preventive Veterinary Medicine;

【通讯作者】 Shi Qiumei;

【机构】 Changli Bureau of Agriculture,Forestry, Animal Husbandry and Aquatic ProductsQilu Shenghua Pharmaceutical Co., Ltd.Animal Husbandry Bureau of Haigang District,QinhuangdaoLeting Animal Husbandry BureauLiushouying Animal Epidemic Surveillance Station, Agriculture and Animal Husbandry Bureau of Funing DistrictAnimal Epidemic Prevention Station of Luanzhou Town, Luanzhou Bureau of Animal Husbandry and Aquatic ProductsHebei Animal Husbandry StationHebei Normal University of Science and Technology/Hebei Key Laboratory of Preventive Veterinary Medicine

【摘要】 In order to isolate and identify the pathogenic bacteria causing dead chickens in a chicken farm in Qinhuangdao area, the liver, heart and other organs of dead chickens suspected of salmonella disease were collected aseptically, and streaked on SS agar medium and chromagar medium. Transparent colonies were observed on SS agar medium, and purple and transparent colonies on CAS medium. The isolate was conducted purification, staining microscopy, biochemical tests, and 16 S rRNA sequence analysis, and the results showed that four strains of the isolated bacte-ria were salmonella. The 16 S rRNA sequence analysis of four strains of salmonella showed that the isolates shared more than 99% homology. Drug susceptibility test was performed using paper method, and the results showed that most of the strains were resistant to tilmicosin, cefradine and sul-famethoxazole, but were sensitive to ceftriaxone.

【基金】 Supported by The Third Batch Giant Plan of Hebei Province(180416H)
【所属期刊栏目】 Animal Disease and Drug (2019年01期)
  • 【DOI】10.19578/j.cnki.ahfs.2019.01.012
  • 【分类号】S852.61
  • 【下载频次】9
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