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Hippocampal Neuron-Derived Extracellular Matrix Coated Nanofibrous Scaffold for Neural Tissue Engineering

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【作者】 郜增刘月王冲李月霞施剑敏汤赋匀娄向新

【Author】 GAO Zeng;LIU Yue;WANG Chong;LI Yuexia;SHI Jianmin;TANG Fuyun;LOU Xiangxin;College of Chemistry,Chemical Engineering and Biotechnology,Donghua University;

【通讯作者】 娄向新;

【机构】 College of Chemistry,Chemical Engineering and Biotechnology,Donghua University

【摘要】 The aim of this study is to prepare poly-L-lactide( PLLA) electrospun nanofibrous scaffolds coated with hippocampal neuron-derived extracellular matrix( N-ECM)and construct a novel neural tissue engineering scaffold.Neonatal rat hippocampal neurons were seeded on PLLA nanofibers,and then decellularized to derive a cell-free extracellular matrix loaded N-ECM/PLLA modified scaffolds. The morphology and ingredients of N-ECM/PLLA were observed by scanning electron microscopy( SEM) and immunofluorescence staining respectively, and the cytocompatibility of the composite scaffolds was characterized by cell count kit-8( CCK-8) assay. The N-ECM was clearly identified loading on scaffolds when being imaged via SEM and immunofluorescence staining results showed that the N-ECM was made up of fibronectin and laminin. Most importantly, compared with tissue culture polystyrene and pure scaffolds, N-ECM/PLLA scaffolds could effectively facilitate the proliferation of rat adrenal neuroma cells( PC12 cells),indicating their better cell compatibilities. Based on the combination of N-ECM and PLLA biomaterials,the present study has fabricated a unique and versatile neural tissue engineering scaffold,offering a new thought for future neural tissue engineering.

【基金】 Fundamental Research Funds for the Central Universities,China(No.16D110520)
  • 【分类号】TQ342.87;TB383.1
  • 【下载频次】3
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