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Cloning and Expression of Extremely Heat-Resistant Endo-β-1,4-Glucanase Gene from Thermotoga maritima in Bacillus subtilis

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【作者】 郝亚荣陈婷张兴群

【Author】 HAO Yarong;CHEN Ting;ZHANG Xingqun;Key Laboratory of Science & Technology of Eco-Textile,Ministry of Education;College of Chemistry,Chemical Engineering and Biotechnology,Donghua University;

【通讯作者】 张兴群;

【机构】 Key Laboratory of Science & Technology of Eco-Textile,Ministry of EducationCollege of Chemistry,Chemical Engineering and Biotechnology,Donghua University

【摘要】 Gene encoding endo-β-1,4-glucanase( TM1525) is derived from Thermotoga maritima( T. maritima),which has an open reading frame of 825 bp and encodes a 274 amino acid endo-β-1,4-glucanase. This enzyme has the same high temperature resistance as thermophilic bacteria,which is an ideal property for industrial applications. By molecular biological means,TM1525 was cloned into p HT43 vector and introduced into Bacillus subtilis( B. subtilis) WB800 N by electroporation. The results showed that the WB800 N expression system was successfully constructed, and extracellular expression of the recombinant gene was achieved. Cellulose hydrolyzed activity of the protein was exhibited.

【基金】 National Natural Science Foundation of China(Regional Fund)(No.51863020)
  • 【分类号】Q78
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