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Identification and LuxAB Biochemical Mark of A Certain Bacterium Contributing to Settle Continuous Cropping Obstacles of Rehmannia glutinosa

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【Author】 TAN Ya-nan1,2, MA Hui-quan1*, WEN Xue-sen2, LIU Shu1 1. School of Life Sciences, Shandong University of Technology, Zibo, Shandong 255049, China; 2. College of pharmacy, Shandong University, Jinan, Shandong 250100, China

【摘要】 [Objective]The research aimed at studying the characteristics and its effects of a certain bacterium which was benefit to settle continuous cropping obstacles of Rehmannia glutinosa. [Method]Combining with morphological observation, gram stain and comparison of physiological and biochemical characteristics, conservative sequence alignment of bacterial 16S rDNA was used to identify the No. 43 strain. The recombinant strain was obtained by marking with LuxAB, antibiotic selection and caprimadehyde detection in the dark room. Finally, the effects of No. 43 and No. C-43 strain on cultured Rehmannia glutinosa seedlings in the medium including dried rhizome of Rehmannia glutinosa extracts were studied. [Result]No. 43 strain was Pseudomonas sp., and the characteistics of No. 43 strain and the recombinant No. C-43 strain were similar, which revealed that No. C-43 strain was still benefit to settle continuous cropping obstacles of Rehmannia glutinosa. [Conclusion]The recombinant No. C-43 strain can be used for the following study of the field experiments.

【基金】 Supported by National Eleventh-Five Year Research Program(2006BA109B03);National Natural Science Foundation of China(30572326)
  • 【分类号】S567.234
  • 【被引频次】3
  • 【下载频次】60
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