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Study on Genomic DNA Extraction Method for Sophora Tonkinensis Gagnep. from Genuine Medicinal Material in Guangxi

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【Author】 TANG Mei-qiong, WEI Fan, LI Lin-xuan, HUANG Yong-cai, QING Liu-yan, MIAO Jian-hua* Guangxi Botanical Garden of Medicinal Plants, Nanning, Guangxi 530023, China

【摘要】 [Objective] The aim was to develop a simple and rapid method to extract the high-quality genomic DNA of Sophora Tonkinensis Gagnep. from genuine medicinal material in Guangxi. [Method] The modified Cetyltrimethyl Ammonium Bromide (CTAB) method was used. [Result] The high-quality and unbroken DNA was obtained using this method by which the interfering substances (i.e. polysaccharides) were eliminated. The obtained DNA can be digested completely by restriction enzymes of EcoRI and MseI, and the clear polymorphism band was gained by AFLP analysis. [Conclusion] This method is suitable for the extraction of genomic DNA in Sophora Tonkinensis Gagnep. and the DNA yielded can be further used for molecular biology experiments.

【基金】 Supported by Natural Science Foundation of Guangxi Province(0991025Z)
  • 【分类号】R284
  • 【被引频次】3
  • 【下载频次】35
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