LOU Fang-ming1*, WANG Yan2, Qian Jing1 1. Zunyi Medical College, Zunyi, Guizhou 563003, China; 2. Hubei University for Nationalities, Enshi, Hubei 445000, China.
【摘要】 [Objective] The aim was to investigate the technological parameters of the refining process of Icraiin from Epimedium wushanense T. S. Ying by polyamide. [Method] Polyamide chromatogram was adopted to separate and purify. Gradient elution used 10%-40% alcohol, and HPLC was adopted to undertake assaying of the Icraiin. [Result] The content of the Icraiin by adopting the gradient elution with 30% alcohol was 17.22%. Total yield reached 95.97%. [Conclution] The method is simple to operate and has good reproducibility. It can purify and enrich the Icraiin from E. wushanense effectively.更多还原