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Study on Purification of Hydroxy Safflower Yellow A From Carthamus tinctorius L. by Macroporus Resins

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【Author】 ZHANG Liang, LIN Peng-cheng*, LI Hu-ye, MA Xue-hua Department of pharmacy, Qinghai Nationalities University, Xining, Qinghai 810007, China

【摘要】 [Objective]The research aimed at obtaining the optimum conditions for the isolation and purification of hydroxy safflower yellow A (HSYA) from Carthamus tinctorius L. extracts using macroporous resins and providing basis for the effective industrial production of HSYA. [Method] Resins were screened through the determination of adsorption ratio and desorption ratio of HSYA. The yield and purity of HSYA were used as indices to investigate the purification conditions including the rate of adsorption, pH and concentration of test solution ass well as the flow rate and concentration of the eluent etc. [Result]With HPD-400 as the adsorbed resin, 50% ethanol as the eluent, adsorption rate of 6 BV/h, and the eluent flow rate of 8-10 BV/ h, the purification efficiency was the best, under which the yield and the purity of HSYA were 73.8% and 35.7% respectively. [Conclusion] The purification efficiency of HSYA is promoted greatly by purifying, which will provide the theoretical evidence for effective industrial production of Carthamus tinctorius L.

【基金】 Supported by Study on Isolation Technology of HSYA from " Hehuang red flower"(2008-G-135)
  • 【分类号】TQ461
  • 【被引频次】4
  • 【下载频次】46
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