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Determination of Total Flavonoids in Limonium aureum(L.) Hill.by Spectrophotometry

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【Author】 LIU Yu1*,ZHANG Ying-peng2,LIANG Jian-ping1,HUA Lan-ying1,WANG Xue-hong1,SHANG Ruo-feng1 1.Lanzhou Institute of Animal and Veterinary Pharmacological Sciences,Chinese academy of Agricultural Sciences,Lanzhou,Gansu 730050,China;2.Institute of Petrochemical Technology,Lanzhou University of Science and Technology,Lanzhou,Gansu 730050,China

【摘要】 [Objective] The aim was to establish a simple and quick method to determine total flavonoids content in Limonium aureum(L.) Hill.and provide scientific evidence for the quality control and development of Limonium aureum(L.) Hill.[Methods] Quercetin as reference substance,UV spectrophotometry was adopted in this study and the detection wavelength was 370 nm.[Results] The linear relationship was good(r=0.999 6) in the range of 2.5-12.5 μg/ml,the average recovery rate of total flavonoids was 99.46% and the content of total flavonoids in the samples was 1.63%.[Conclusion] UV spectrophotometry is a suitable method for detecting total flavonoids content in Limonium aureum(L.) Hill.with the advantages of simple and quick which would provide the scientific evidence for the quality control and development of Limonium aureum(L.) Hill.

【基金】 Supported by Special Fund of State Level Commonweal Scientific Research Institute (BRF080401)
  • 【分类号】S567.239
  • 【被引频次】6
  • 【下载频次】48
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