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Isolation of Genomic DNA in Morinda citrifolia Linn.and Establishment of Its ISSR-PCR Reaction System

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【Author】 WU Tian1,LAN Zeng-quan2*,LI Qing-hong2 1.Key Laboratory of Landscape Plants and Ornamental Horticulture,Southwest Forestry University,Kunming 650224,China;2.Environment Science and Engineering Department,Southwest Forestry University,Kunming 650224,China

【摘要】 [Objective] The research aimed to isolate the genomic DNA in Morinda citrifolia Linn.and establish its ISSR-PCR reaction system.[Method] The Morinda citrifolia Linn.leaves of 3 germplasm resources from Hainan and 4 germplasm resources from America were chosen as test materials,the isolation of DNA in Morinda citrifolia Linn.and establishment of ISSR-PCR were studied.[Result] The results showed that DNA of Morinda citrifolia Linn.leaves with high quality were gained by the modified CTAB method.The isolated DNA could be used for ISSR analysis and 7 primers were found to amplify polymorphic products.[Conclusion]The rapid and efficient extraction method and the ISSR analysis procedure in this experiment have laid a good foundation for application of ISSR in Morinda citrifolia Linn.genetic research.

【基金】 Supported by "948" Project of State Forestry Administration,P. R.China (2007-4-08);Key Foundation of Landscape Plants and Ornamental Horticulture of Yunnan Province (500974)
  • 【分类号】S567.239
  • 【被引频次】12
  • 【下载频次】15
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