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Determination of Seven Metabolites of Magnolia officinalis Rendet Wils.by HPLC

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【Author】 FANG Xiao-ping1,YI Yin,YU Zheng-wen1,YANG Zhan-nan2,HU Guang-ping1,2 1.School of life science,Guizhou Normal University,Guiyang,Guizhou 550001,China;2.Key Laboratory for Information System of mountainous Area and protection of Ecological Environment of Guizhou Province,Guizhou Normal University,Guiyang,Guizhou 550001,China

【摘要】 [Objective] To establish an optimal extraction process for simultaneous determination of seven effective metabolites of Magnolia officinalis Rendet Wils.By HPLC method.[Method] The chromatographic conditions were as follows:Samples were performed on the chromatographic column model Shim-pack CLC-ODS(4.6 mm×250 mm,5 m).The mobile phase consisted of acetonitrile(A) and 0.1% formic acid(B).[Result] The regression equation,with peak area(y) as ordinate and concentration(x) as abscissas,of chlorogenic acid,rutin,hyperin,quercitrin,quercetin,magnolol and honokiol,were y =1E+05x-306 360(r=0.999 8),y=5E+04x-79 920(r=0.999 4),y=5E+04x-55 280(r=0.999 9),y=5E+04x-60 530(r=0.999 9),y=2.5E+04x-57 010(r=0.999 8),y=2E+04x+7 102(r=0.999 9) and y=2E+04x+7 102(r=0.999 9),respectively.The concentrations of reference substances showed good linear relationship with peak area within 3.125-100.00 μg/ml.The average recovery rates of chlorogenic acid,rutin,hyperin,quercitrin,quercetin,magnolol and honokiol were 103.98%,101.65%,99.17%,103.59%,103.59%,102.44% and 104.62%,with RSD of 3.37%,2.86%,3.02%,2.89%,2.86%,1.18% and 3.67%,respectively.The content of chlorogenic acid,rutin,hyperin,quercitrin,quercetin,magnolol and honokiol from different parts of Magnolia offiinalis were different from each other.All the 7 effective compounds could be detected in the leaf of Magnolia offiinalis,thereinto,the content of quercitrin,magnolol and honokiol were high,while the content of hyperin and quercetin were low.[Conclusion] The established HPLC method was simple,convenient,stable and with good repeatability,which would provide reference for the further development and utilization of Magnolia officinalis Rendet Wils.

【基金】 Supported by Scientific and Technological Project in Guizhou Province (06-0502zy015)
  • 【分类号】R284;O657.72
  • 【被引频次】1
  • 【下载频次】34
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