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Rapid Determination of Quercetin Content in Gynostemma pentaphyllum(Thunb.) Makino by HPLC

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【Author】 WANG Shao-yun,ZHANG Wen-hua,JIANG Tian-zhi,XIAO Zhi-you College of Environment and Life Science of Kaili University,Kaili,Guizhou 556000,China

【摘要】 [Objective] To establish a simple,rapid and accurate analytical method for detecting quercetin in Gynostemma pentaphyllum(Thunb.) Makino by high perfomance liquid chromatograph(HPLC).[Method]The conditions of chromatography were as follows:Microsorb-MV100-5C18 column(4.6 mm × 250 mm,5 m) was adopted with a mobile phase of methanol-mixtrue [acetonitrile:1% acetic acid:0.4% phosphoric acid,V/V/V](37:63,V/V);the flow rate was 0.6 ml/min,and the detective wavelength was 372 nm.[Result]The linear range was 0.64-25.60 g/ml(r = 0.999 9),the average recovery was over 96%(n = 9).[Conclusion] The established method is simple,rapid and accurate.

【基金】 Supported by Research Projects of Kaili University(z0909);Key Discipline Construction of Botany of Kaili University
  • 【分类号】R284;O657.72
  • 【被引频次】3
  • 【下载频次】32
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