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Optimization of Separation Condition of Triterpenoids from Ganoderma lucidum under HPLC

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【Author】 DING Fang-lin Hunan Biological and Electromechanical Polytechnic,Changsha,Hunan 410127,China

【摘要】 [Objective]To optimize the separation condition of triterpenoids from Ganoderma lucidum.[Method]The effects of different extraction solvents,different mobile phases and different sampling volume on the separation of triterpenoids were studied by RP-HPLC,and the triterpenoids of 4 kinds of health care products were determined under the optimum condition.[Result]The optimum condition was as follows:extraction solvents of chloroform,mobile phase of 0.2% acetic acid-water,sampling volume of 10-30 μl,and the chromatogram of 2 kinds of the health care products were similar with that of Ganoderma lucidum medicinal material.[Conclusion]The optimum conditions were obtained,and the 4 kinds of health care products were determined by this method,which could used to control the extraction quality of Ganoderma lucidum and evaluate the health care products.

【基金】 Supported by University Research Project in Hunan Province in 2009 (09C1212)
  • 【分类号】S567.31
  • 【下载频次】34
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