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Comparative Study on the Preservation Methods of Cordyceps sinensis Culture

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【Author】 FANG Su*, LIN Qin, NI Zhi-jing College of Life Science and Engineering, Northern University for Nationalities, Yinchuan, Ningxia 750021, China

【摘要】 [Objective]To compare the preservation effects of three long-term preservation methods on inhibiting the metabolic activity of Cordyceps sinensis culture.[Method] Taking the growth of mycelium,polysaccharide of and cordycepic acid as indexes to evaluate quality of Cordyceps sinensis,the effects of sharp freezing,liquid nitrogen freezing and freeze-drying on conserving Cordyceps sinensis culture of C-03 were comparatively studied.[Result]The Cordyceps sinensis culture through vacuum freeze-drying preservation due to skimmed milk and phased cooling of 4 ℃ for 30 min,-20 and-80 ℃ freezing for 2 and 24 h respectively was growing well when germination,the yield of mycelial growth and active ingredients were relatively higher than the other two methods.[Conclusion]Vacuum freeze-drying preservation was the desirable method to long-term preserve Cordyceps sinensis culture.

【基金】 Supported by the Key Laboratory Project of State Nationalities Affairs Commission(2007SY005);Research Project of Northern University for Nationalities(2004Y031)
  • 【分类号】S567.35
  • 【被引频次】2
  • 【下载频次】82
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