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Identification of No.1 and Male Sterility of Lycium barbarum L. by ISSR-PCR Molecular Marker Technique

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【Author】 SI Bin-bin*, WANG Zhen College of Bioscience and Engineering, Northern University for Nationalities, Yinchuan, Ningxia 750021, China

【摘要】 [Objective]To identify genuine medicinal materials Lycium barbarum L.by ISSR-PCR molecular marker technique on the level of DNA molecular.[Method]Suitable primers were screened out from 50 ISSR primers(Inter-simple sequence repeat),and then used in PCR amplification and electrophoretic analysis for distinguishing Lycium barbarum L.from No.1 and male sterility by finding specific sites.[Result]1 ISSR primer could amplify polymorphic bands that suitable for distinguishing Lycium barbarum L.from No.1 and male sterility.[Conclusion]ISSR-PCR molecular marker technique was quick and reliable,which could be used in identification of Lycium barbarum L..

【基金】 Supported by Project of Northern University for Nationalities(2007Y049)
  • 【分类号】S567.19;R440
  • 【被引频次】5
  • 【下载频次】34
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