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Establishment of HPLC Fingerprint of FRUCTUS EVODIAE and Determination of Three Components

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【Author】 ZENG Jin-xiang1,LIU Yong1,HUANG Bi-tao2,YANG Ya-qin1*,ZHU Ji-xiao1,ZHANG Zhong-li1,LUO Guang-ming1,XIONG Hong-hong1 1.Chinese Medicine Germplasm Resource Engineering Technology Research Center of Jiangxi Province,Jiangxi University of Traditional Chinese Medicines,Nanchang,Jiangxi 330004,China;2.Hongdu Hospital of Traditional Chinese Medicine of Nanchang,Nanchang,Jiangxi 330008,China

【摘要】 [Objective] To establish the fingerprint of FRUCTUS EVODIAE and determine the contents of chlorogenic acid,hyperoside and evodiamine simultaneously.[Method]The chromatographic separation was performed on Hypersil C18 column(250.0 mm×4.6 mm,5 μm) with mobile phase of acenitrile-1% acetic acid in gradient elution whose flow rate was 1.0 ml/min.Then samples with sampling volume of 10 μl were detected at the wavelength of 270 nm.[Result] There were 19 common peaks in the fingerprint of 11 batches of FRUCTUS EVODIAE with the RSD value of the relative retention time and peak area less than 2.50% and 2.10%,respectively.In general,FRUCTUS EVODIAE made in Jiangxi Province was higher in contents of chlorogenic acid,hyperoside and evodiamine than that produced in Guizhou and Yunnan Province.[Conclusion] The fingerprint and simultaneous determination of chlorogenic acid,hyperoside and evodiamine in FRUCTUS EVODIAE were firstly studied,which provided better means for the quality control of FRUCTUS EVODIAE produced in different places and different harvesting time,and provided technique support for GAP cultivation of FRUCTUS EVODIAE.

【基金】 Supported by Young Teacher Training Program Funded Projects of Key Disciplines of Jiangxi University of Traditional Chinese Medicines(2010023)
  • 【分类号】R284;O657.72
  • 【被引频次】3
  • 【下载频次】28
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