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Determination of Amino acid in Flower of Lilium brownii var. viridulum by Pre-column Derivation OPA-HPLC

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【Author】 CHEN Li-de1,LIU Xin-tao2,JIANG Sheng-yan1,WEI Zhao-an1,ZHANG Za-ru3 1.Department of Biological and Chemical Engineering,Shaoyang University,Shaoyang,Hunan 422000,China;2.Hunan Green Foodstuff Office,Changsha,Hunan 410005,China;3.Department of Basic Medical,Yueyang Vocational Technical College,Yueyang,Hunan 414000,China

【摘要】 [Objective] To determine the contents of amino acids in flower of Lilium brownii var.viridulum.[Method] Contents of 17 amino acids in flower of Lilium brownii var.viridulum were determined using OPA-HPLC method,in which o-phthalaldehyde(OPA)and 9-fluore-nylmetloy chorofomnate(FMOC)were selected as pre-column derivative reagent and VWD detector was used.[Result] The Lilium brownii var.viridulum from Shaoyang was rich in Asp,Glu,Ser,His,Gly,Thr,Arg,Ala,Tyr,Cys,Val,Met,Phe,Iso,Leu,Lys and Pro.The contents of essential amino acids for adult and for children were 49.7 and 30.6 mg/g,respectively,which were 31.20% and 19.20% of total contents of amino acids individually.Content of Glu and Arg in samples ranked the first and second,respectively,which were 28.7 and 27.5 mg/g respectively,taking up 18.03% and 17.30% of total amino acids.[Conclusion] The method does not require proprietary analyzers and is simple in operation,high in accuracy and accurate in determining result.Flower of Lilium brownii var.viridulum is rich in amino acids,which is of high developing value.

【基金】 Supported by Planning Project from Hunan Science and Technology Bureau(2010NK3041)
  • 【分类号】S682.29
  • 【被引频次】1
  • 【下载频次】69
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