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Study on the Extraction Technology of Chlorophyll from Leaves of Brassica juncea var.tumida Tsen et Lee by Pectinase-assisted Method

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【Author】 LI Min Life Science and Technology College,Yangtze Normal University,Fuling,Chongqing 408000,China

【摘要】 [Objective] To optimize the extraction technology of chlorophyll from fresh leaves of Brassica juncea var.tumida Tsen et Lee by pectinase-assisted method.[Method] Effects of enzymolysis temperature,ethanol volume fraction,extraction time and volume of extracting solvent on the extraction of chlorophyll from fresh leaves of Brassica juncea var.tumida Tsen et Lee by pectinase-assisted method were investigated through single factor test and quadratic regression rotation-orthogonal combination design.[Result] The optimum extraction conditions were as follows:enzymolysis temperature of 10 ℃,ethanol volume fraction of 40%,extraction time of 60 min,and volume of extracting solvent of 70 ml.The theoretical extraction rate of chlorophyll was 32.29 mg/g which was approximated to the experimental value of 31.87 mg/g.[Conclusion] This study could provide reference for rational development and utilization of Brassica juncea var.tumida Tsen et Lee and improvement of extraction rate of chlorophyll.

【基金】 Supported by the Science and Technology Project of Chongqing Education Commisson(KJ101316)
  • 【分类号】S637.3
  • 【下载频次】40
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