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Determination of Protocatechuic Acid in CORTEX ACAN THOPANACIS by High Performance Liquid Chromatography-Four Channel UV-Vis Detection Method

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【Author】 CHEN Fang1,FENG Li-juan2 1.Department of Chemistry and Environmental Engineering,Jianghan University,Wuhan,Hubei 430056,China;2.Department of Bioengineering,Zhuhai Campus of Zunyi Medical College,Zhuhai,Guangdong 519041,China

【摘要】 [Objective] To establish a method to determine the content of protocatechuic acid in CORTEX ACAN THOPANACIS.[Method] Content of protocatechuic acid in CORTEX ACAN THOPANACIS was determined by high performance liquid chromatography-four channel UV-Vis detection method.Chromatographic conditions were as follows:ODSC18 chromatographic column(250 mm×4.6 mm,5 μm) ,and buffer solution of methanol and sodium dihydrogen phosphate as the mobile phase(0.1 mol/L,15∶85,V/V,pH=2.5) .4 detection wavelengths were 245,260,275 and 290 nm with the quantitative detection wavelength of 260 nm.[Result] Protocatechuic acid had good linear relationship within the range of 0.5-6.0 mg/L(r=0.999 9) .The average recovery rate was 99.87% with RSD=2.11%.[Conclusion] The method was reliable,stable,and simple,and could be used for the content determination of protocatechuic acid in CORTEX ACAN THOPANACIS.

  • 【分类号】R284
  • 【被引频次】1
  • 【下载频次】25
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