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Extraction and Determination of Emodin in Polygonum perfoliatum L.

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【Author】 ZOU Deng-feng1,XIE Ai-ze2,3,QIU Yu-ting1,YE Kai1,CHEN Yi1,ZHANG Yan1 1.School of Pharmacy,Guilin Medical University,Guilin,Guangxi 541004,China;2.Guangxi Institute of National Medicinal Research,Nanning,Guangxi 530001,China;3.First Affiliated Hospital of Guangxi Medical University,Nanning,Guangxi 530022,China

【摘要】 [Objective] To extract and determine the content of emodin in Polygonum perfoliatum L.[Method] The content of emodin was determined by reverse phase HPLC.A column of Kromasil C18 was used and a mixture of methanol-water-glacial acetic acid(87∶13∶215) was used as the mobile phase at a flow rate of 110 ml/min.The UV detection wavelength was 254 nm and the column temperature was room temperature.[Result] The emodin showed a good linear relationship within the range of 1-40 μg/ml(r=0.999 9) ;the linear regression equation was Y=112.89X-8.912(r=0.999 9) .The optimum extraction technology was 80% ethanol concentration,35 ml ethanol dosage and 2 h extraction time.The average recovery rate was 99.16%(RSD=1.49%) .[Conclusion] The method was simple,rapid and accurate.It could be used for the extraction and content determination of emodin from Polygonum perfoliatum L.

【基金】 Supported by the Science and Technology Project of Traditional Chinese Medicine by Guangxi Administration of Traditional Chinese Medicine(GZ-KZ09-52);the Project of Education Department of Guangxi
  • 【分类号】R284
  • 【下载频次】30
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