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Induction of Leaf Callus and Detection of Stilbene Glucoside Content in RADIX POLYGONI MULTIFLORI

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【Author】 FAN Hong-qiong* Chongqing Three Gorges Polytechnic College,Wanzhou,Chongqing 404001,China

【摘要】 [Objective] To carry out the induction of leaf callus and the detection of stilbene glucoside content in RADIX POLYGONI MULTIFLORI.[Method] Under dark culture conditions at 25 ℃,leaves of RADIX POLYGONI MULTIFLORI were cultured on MS medium(1 mg/L 2,4-D + 0.4 mg/L NAA) for 15 d.Then,the calluses induced were transferred to the same MS medium for 3 times,with 15 d for each subculture.Content of stilbene glucoside in leaf callus was detected by HPLC.[Result] Calluses induced grew well,and the content of stilbene glucoside in leaf callus was 0.37 mg/g.[Conclusion] There was stilbene glucoside in leaf callus of RADIX POLYGONI MULTIFLORI.And the leaf callus could be used for the further physiological and biochemical studies.

  • 【分类号】R284
  • 【被引频次】2
  • 【下载频次】34
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