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Study on the Preparation Technique on Synthetic Seeds of Atractylodes macrocephala Koidz.

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【Author】 WANG Fu-yuan1,TANG Ning2*,BEN Ai-ling2 1.Higher Vocational and Technical School,China Pharmaceutical University,Nanjing,Jiangsu 211198,China;2.School of Biochemistry and Environmental Engineering,Nanjing Xiaozhuang College,Nanjing,Jiangsu 211171,China

【摘要】 [Objective] To study the best synthetic endosperm combination of Atractylodes macrocephala Koidz.seeds and to provide the theoretical basis for development and large-scale application of A.macrocephala synthetic seeds.[Method] Factors to affect germination rate and seedling conversion rate,such as encapsulated propagule and synthetic endosperm components were studied with germfree tube plantlet system of A.macrocephala.[Result] The germination rate and seedling conversion rate of synthetic seeds could reach 100% and 92% with auxilizry buds,2.5% sodium alginate + 2% CaCl2 as synthetic endosperm encapsulation,and 0.5 mg/L IAA and 30 g/L sucrose as synthetic endosperm components.[Conclusion] The technique of rapid multiplication of synthetic seeds on the basis of tissue culture of A.macrocephala has definite superiority.

【基金】 Supported by the Special Fund for Youth of Nanjing Xiaozhuang College (2008NXY46)
  • 【分类号】S567.233
  • 【下载频次】25
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