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Study on the Extraction of Huperzine A from Huperzia serrata by Enzymatic Method

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【Author】 YI Xue-wen* ,ZHANG Li,LUO Rong-zhen,DU Ping Sichuan University of Science & Engineering,Zigong,Sichuan 643000,China

【摘要】 [Objective]To screen the optimum extraction technology of huperzine A from Huperzia serrata by enzymatic method. [Method]Using cellulase to hydrolyze the cytoderm,orthogonal test was carried out to optimize the extraction technology of huperzine A from H. serrata by enzymatic method. [Result]The optimum extraction technology of huperzine A from H. serrata by enzymatic method was a combination of 0.125 g cellulase,4.5 pH,60 ℃ enzymolysis temperature and 2.5 h enzymolysis time,under which the extraction rate of huperzine A could reach 0. 589‰. Compared with the acidsoak method,the extraction rate of huperzine A by enzymatic method increased by 40.3%. Besides,enzymolysis temperature was the biggest influence factor to the extraction of huperzine A from H. serrata by enzymatic method. [Conclusion]This method was simple, convenient and efficient,which could be used for extracting huperzine A from H. serrata.

  • 【分类号】TQ461
  • 【下载频次】69
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