节点文献

Trace Analysis on Aristolochic acid A in RADIX ET RHIZOMA ASARI from Different Production Areas

免费订阅

【Author】 YAN Jing-sen1*,LI Jing-hui2,SUN Guo-jun1,LIU Feng-yun2 1.Department of Medicinal Chemistry,Liaoning Institute of Science and Technology,Benxi,Liaoning 117004,China;2.Liaoning Benxi Institute for Drug Control,Benxi,Liaoning 117000,China

【摘要】 [Objective] To determine and compare the content of aristolochic acid A in RADIX ET RHIZOMA ASARI from 12 different production areas by establishing Reversed-Phase High Performance Liquid Chromatography(RP-HPLC).[Method] The content of aristolochic acid A in the extract of RADIX ET RHIZOMA ASARI prepared by ultrasonic extraction was determined by RP-HPLC.RP-HPLC used the chromatographic column Zorbax SB-C18(4.6 mm ×150 mm,5 μm) with the detection wavelength at 316 nm.Following the injection of 5 μl of sample solution at room temperature,an elution with acetonitrile-water solution(V/V,50:50) as mobile phase was initiated at a flow rate of 1.0 ml/min.[Result] Except the RADIX ET RHIZOMA ASARI in Fengcheng,no aristolochic acid A was detected.The content of aristolochic acid A in RADIX ET RHIZOMA ASARI produced in Fengcheng showed good linear relationship within the range of 2.04 to 20.4 ng/ml.[Conclusion] This method is rapid and simple with high recovery rate and good linear relationship,sensitivity and repeatability.Thus,it could be used for the determination of aristolochic acid A content in RADIX ET RHIZOMA ASARI.And the RADIX ET RHIZOMA ASARI used in this research had relatively high quality.

【基金】 Supported by the Science and Technology Research Project of Liaoning Provincial Education Department (2009A382)
  • 【分类号】TQ461
  • 【下载频次】17
节点文献中: 

本文链接的文献网络图示:

浏览历史:
下载历史: